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Common symptoms of a vitamin B12 deficiency include: Tiredness Lethargy Feeling faint Breathlessness Less common symptoms include: Headaches Heart palpitations Altered taste Loss of appetite Ringing in the ears (tinnitus) Looking pale Sore mouth and tongue If it is left untreated you can end up with longer term problems including: Psychological problems such as depression, confusion, memory problems Nerve problems such as numbness, pins and needles, vision changes and unsteadiness A blood test can indicate if you have a B12 deficiency

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Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP