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Preparation of MRS without glucose The MRS medium was prepared using the following ingredients (g/L): peptone 10.0, beef extract 8.0, yeast extract 5.0, polysorbate 80 1.0, ammonium citrate 2.0, sodium acetate (anhydrous) 3.0, magnesium sulfate 0.1, manganese sulfate 0.05, and dipotassium phosphate 2.0, without glucose
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