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The medium was inoculated with 4 × 10 4 yeast cells and incubated at 27 °C for 24 h, before samples containing 1 × 10 6 cells were collected from the suspension, quenched with an equal volume of cold (–80 °C) 100% methanol and incubated with a 100 μm solid-phase microextraction fibre coated with polydimethylsiloxane (Supelco, Sigma-Aldrich, St Louis, MO, USA) for 30 min
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At the least, peptides made by the proto-ribosome might simply have augmented the ancestral PTC
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