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Assembly of the middosome induces association and activation of the E3 ubiquitin ligase TNF receptor-associated factor 6 (TRAF6) which can trigger several different pathways (Pereira and Gazzinelli, 2023)

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For Dry Eye-Related Twitching Preservative-free lubricating eye drops (used 3–4 times daily) Warm compresses and eyelid hygiene Omega-3 fatty acid supplementation In more severe cases, prescription anti-inflammatory eye drops For Allergic Twitching Antihistamine eye drops Cold compresses Avoiding the allergen trigger (dust, pollen, pet dander) Oral antihistamines if systemic allergy symptoms are present For Benign Essential Blepharospasm Botulinum Toxin (Botox) injections are the gold-standard treatment