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Upon treatment of strep-MBP-ROXY9 with 10 mM DTT and subsequent alkylation of the TCA-precipitated protein in the presence of 1% SDS, the mobility of the protein was reduced due to the addition of mmPEG to the five reduced cysteines in the ROXY9 moiety of the protein (Fig

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Now I'm not familiar and I definitely haven't delved into the science of everything -- Dave Asprey: [00:42:19] You don’t need to