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The liver mitochondrial preparation (1.0 mg/ml) was incubated with sertraline (37.5, 50, 75, and 100μM) in a reaction buffer containing 210mM mannitol, 70mM sucrose, 2μM rotenone, and 5mM HEPES, pH 7.4 at 30°C in the presence of 20μM CaCl 2
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