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Cells were incubated for 2 h at RT with a primary antibody mixture of polyclonal guinea pig anti-Synaptophysin-1 (1:1000, SYSY), polyclonal chicken anti-MAP2 (1:10,000, Abcam), and polyclonal rabbit anti-Munc18-1 antibodies (1:1000, described previously

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However, not enough is known about this theoretical issue to elucidate a risk-benefit tradeoff and how timing of treatment works into such a tradeoff