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Additionally, ROS-induced oxidative modifications of cellular macromolecules generate damage-associated molecular patterns (DAMPs), which are recognized by pattern recognition receptors, such as Toll-like receptors (TLRs), thereby perpetuating sterile inflammatory responses (Koenig and Buskiewicz-Koenig, 2022)

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Cells were then incubated with anti-Nrf2 antibody for 3 hours at room temperature with gentle shaking