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Next, 3 μg of mouse anti-Strep II-Tag mAb or rabbit anti-DDDDK-tag (Flag) mAb, along with rabbit or mouse control IgG, was coupled to the protein A/G beads in 250 μl of IP buffer at 40 °C for 4 h on a rotating wheel
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DESCRIPCIÓN DEL EXPERIMENTO El objetivo de esta práctica es introducir a los estudiantes en los principios de la cromatografía de filtración en gel como un método para separar moléculas en función de su tamaño y forma
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